MicroQuik™

 

Fast Confirmation from First Growth Plate

Rapid Confirmation Tests For:

  • Food Safety
  • Cosmetic Industry
  • Beverages
  • Water Testing
  • Pharmaceutical
   

  • MicroQuik Confirmation Tests will confirm presence or absence of specific organisms from a first growth plate:

– no extra growth time or complicated procedures to do
– saves time!

  • MicroQuik Confirmation Test assays are easier to use and less expensive than the traditional culture methods or real-time PCR, and yields results in less than thirty minutes.
  • MicroQuik Confirmation Test’s ready to use reagents have extended shelf life and room temperature storage.
  • MicroQuik Confirmation Tests are enzymatic assays for the rapid detection of specific micro-organisms.

 

  • Traditional culture confirmation requires not only the initial first growth plate, but subsequent growth plates on specific media.
  • Extra time consisting of many days may be needed to confirm the target microorganism.
     

 

 

MicroQuik unique enzyme detection systems consisting of a synthetic substrate which, in the presence of an enzyme specific to the infectious disease, causes a chemical reaction.

This reaction, when coupled to a developing agent, causes a color to appear on the filter membrane; or is visible under a fluorescent light without the addition of a developing agent.

   

A. Fluorochrome Detection - Detection requiring long wave UV light or reagent.  Results in 5 minutes.

      1.  MicroQuik confirmation of Enterococcus
      2.  MicroQuik confirmation of Streptococcus Group A
      3.  MicroQuik Differnetiation of Gram+/Gram-
      4.  MicroQuik confirmation of Candida Albicans

B. Chromogenic Detection - Detection indicated by a color reaction.  Results in 30 minutes.

      1.  MicroQuik confirmation of E. coli
      2.  MicroQuik confirmation of Total Coliforms

C. Fluorogenic Detection - Detection requiring long wave UV light.  Results in 5 minutes.

      1.  MicroQuik confirmation of Total Coliforms
      2.  MicroQuik confirmation of E. coli and Total Coliforms

 

1. Add 1 drop of Reagent A to the filter membrane.
2. Select colonies that morphologically resemble the target organism.
3. Touch the tops of 2-3 colonies with a loop, inoculating needle, swab, or wooden applicator.  Smear the colonies onto the filter membrane of the test card.


4. Set aside the inoculated card at room temperature for 5 minutes.
5. Add 1 drop of Reagent B to the filter membrane.  A purple color will immediately form on and around the deposited colonies in the presence of the hydrolyzed substrate, indicating a positive test. The test is negative if no purple color appears.  The test result must be read within 1 minute after the addition of Reagent B, otherwise the test result may be invalid.
   
    

 

1.  Add 1 drop of Reagent A to the filter membrane.
2.  Select colonies that morphologically resemble the target organism.
3.  Touch the tops of 2-3 colonies with a loop, inoculating needle, swab, or wooden applicator. Smear the colonies onto the filter membrane of the test card.


4.  Set aside the inoculated card at room temperature for 25 minutes.
5.  After 25 minutes, look for a blue/purple color around the smeared colonies. This is a positive test.  
     
The test is negative if no blue/purple color appears. A positive test is indicative of the target organism.

 

1.  Add 1 drop of Reagent A to the filter membrane.
2.  Select colonies that morphologically resemble the target organism.
3.  Touch the tops of 2-3 colonies with a loop, inoculating needle, swab, or wooden applicator. Smear the colonies onto the filter membrane of the test card.


4.  Set aside the inoculated card at room temperature for 5 minutes.
5.  After 5 minutes, look at the test card under a long wave (360nm) UV light. A positive test will be indicated by a blue/white fluorescence over a blue background around the smeared colonies.




                     
The test is negative if no blue/white fluorescence appears.  A positive test is indicative of the target organism.

 

Vista Enterprises Incorporated (VEI) MicroQuik kits are designed to provide fast confirmation of specific microorganisms.

Because MicroQuik is inexpensive, fast and easy to use, results are obtained in a much more timely manner, thus saving time and losses of product.

VEI can custom design kits for customers that have specific requests such as for a specific bacteria or a particular level of detection.

Please contact VEI if you have such a request.

Vista Enterprises Incorporated (VEI) MicroQuik confirmation kits are currently available for:

  • Enterococcus
  • E. coli
  • Streptococcus Group A
  • Total Coliforms
  • Candida Albicans
  • E. coli and Total Coliforms
  • Gram+/Gram- Differentiation

 

 

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